Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody

Antigen: Actin, alpha 2, Smooth Muscle, Aorta (ACTA2)

Synonyms: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN185271

Additional Information

Alternative name: Smooth muscle alpha-actin

Gene ID: 59

Immunogen: Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1, NP_001135417.1

Format: Affinity-purified

Description: All names and symbols: ACTA2, actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46, smooth muscle alpha-actin,. Official Symbol: ACTA2. RefSeq number(s): NP_001604.1, NP_001135417.1. Gene ontology terms: actin filament, motor activity, muscle development, striated muscle thin filament, structural constituent of cytoskeleton, structural constituent of muscle Variants (NP_001604.1, NP_001135417.1) encode the same protein..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog

Synonyms: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx 45kDa band observed in Human Duodenum and Human Heart lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.1-0.3µg/ml. Immunofluorescence: see publication McIntire RH et al., Placenta. 2008 Jul,29(7):631-8. Epub 2008 Jun 5. (PMID: 18538388).

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Reddy, Ozgur, Lu et al.: "Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region." in: The Journal of biological chemistry, Vol. 265, Issue 3, pp. 1683-7, 1990 (PubMed).