Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody
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| Synonyms |
TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, Casp11, MGC124949, CASP4, CASP13, MGC137679 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Biotin (5), PE (4), FITC (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Application
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Western Blotting (WB) (87), ELISA (36), Immunofluorescence (IF) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Immunohistochemistry (IHC) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunoprecipitation (IP) (4), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN185284 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CASP4
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Gene ID
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837
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Immunogen
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CKERAEEIYPIKERN
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Description
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All names and symbols: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX. Official Symbol: CASP4. RefSeq number(s): NP_001216, NP_150649, NP_150650
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Specificity
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This antibody is expected to recognise all 3 isoforms of CASP4 (alpha, represented by NP_001216, gamma, NP-150649 and delta NP_150650). Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 40kDa band observed in Human heart lysates (calculated MW of 43.3kDa according to NP_001216). Approx 37kDa + 30kDa bands observed in Human Brain lysates (calculated MW of 36.7kDa and 32.7kDa according to NP_150649 and NP_150650 resp.) Recommended concentration: 0.3-2µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).
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Alternatives for antigen "Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4)", type "Antibodies"
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Hosts
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Rabbit (103), Rat (7), Mouse (6), Goat (3), Chicken (2)
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Reactivities
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Human (79), Mouse (Murine) (65), Rat (Rattus) (47), Chicken (19), Zebrafish (18), Cow (Bovine) (4), Monkey (2), Cat (Feline) (1), Dog (Canine) (1)
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Applications
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Western Blotting (WB) (87), ELISA (36), Immunofluorescence (IF) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Immunohistochemistry (IHC) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunoprecipitation (IP) (4), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (5), PE (4), FITC (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Epitopes
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N-Term (12), Internal Region (8), C-Term (3), Middle Region (3), AA 85-101 (2), AA 1-89 (1), AA 119-133 (1), AA 202-217 (1)
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Alternatives 
2013 BioMed Central Research Awards
