ELISA. Western Blot: Casp-4 antibody can be used for the detection of Caspase-4 at 1 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
For Research Use only
PBS, 0.02 % Sodium Azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Avoid repeated freezing and thawing.
4 °C/-20 °C
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease (5).Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX