ADAM33 antibody (ADAM Metallopeptidase Domain 33) (Internal Region)

Details for Product anti-ADAM33 Antibody No. ABIN185401, Supplier: Log in to see
Antigen
  • ADAM13
  • ADAM33
  • Adaml
  • adam13
  • C20orf153
  • DJ964F7.1
  • ADAM metallopeptidase domain 33
  • a disintegrin and metallopeptidase domain 33
  • ADAM33
  • LOC100230755
  • LOC100356023
  • Adam33
  • adam33
Epitope
Internal Region
10
10
3
2
2
1
1
1
1
1
1
Reactivity
Human
53
12
4
4
3
2
2
2
1
1
1
Host
Goat
35
18
15
Clonality
Polyclonal
Conjugate
This ADAM33 antibody is un-conjugated
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
34
20
17
13
6
4
4
1
1
Options
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Immunogen Peptide with sequence CQSRRCRKNAFQEL, from the internal region of the protein sequence according to NP_079496.1.
Blocking Peptide Blocking peptide for this product available: ABIN369714
Specificity This antibody is expected to recognise isoform alpha (NP_079496.1) and not isoform beta (NP_694882.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ADAM33 (ADAM33 Antibody Abstract)
Background Synonyms: ADAM33, a disintegrin and metalloproteinase domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, a disintegrin and metalloprotease 33, disintegrin and reprolysin metalloproteinase family protein, metalloprotease disintegrin, ADAM metallopeptidase domain 33, RP5-964F7.2, C20orf153, MGC149823
Gene ID 80332
NCBI Accession NP_079496
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Experiments gave bands at approx 80-90 kDa and strong ladder from 26 to 38 kDa in Human Prostate lysates after 0.3 µg/mL antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul,31(1):13-21, PMID: 14742294). This protein has a calculated MW of 87.7 kDa according to NP_079496.1. Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-ADAM33 antibody (ADAM Metallopeptidase Domain 33) (Internal Region) (ABIN185401) ABIN185401 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer)...
Background publications Blakey, Halapi, Bjornsdottir, Wheatley, Kristinsson, Upmanyu, Stefansson, Hakonarson, Hall: "Contribution of ADAM33 polymorphisms to the population risk of asthma." in: Thorax, Vol. 60, Issue 4, pp. 274-6, 2005 (PubMed).

Simpson, Maniatis, Jury, Cakebread, Lowe, Holgate, Woodcock, Ollier, Collins, Custovic, Holloway, John: "Polymorphisms in a disintegrin and metalloprotease 33 (ADAM33) predict impaired early-life lung function." in: American journal of respiratory and critical care medicine, Vol. 172, Issue 1, pp. 55-60, 2005 (PubMed).