MDM2 antibody (Internal Region)

Catalog No. ABIN185508

The Goat Polyclonal anti-MDM2 antibody (ABIN185508) specifically detects MDM2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for MDM2 antibody (Internal Region) (ABIN185508)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MDM2 Antibody

Binding Specificity: Internal Region

Purpose: MDM2 (isoform)

Sequence: DELSGERQRK RHKSD

Specificity: This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.

Cross-Reactivity: Cat, Cow, Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Liver lysates (calculated MW of 56.0 kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48 kDa, 60KDa, 70 kDa and 90 kDa are observed. These bands are not obser

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background: MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Md

Gene ID: 4193

NCBI Accession: NP_002383

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway