Mdm2-Binding Protein (MDM2) antibody

Antigen: Mdm2-Binding Protein (MDM2)

Synonyms: HDMX, hdm2, MGC5370, MGC71221

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185508

Additional Information

Alternative name: MDM2 (isoform)

Gene ID: 4193

Immunogen: C-DELSGERQRKRHKSD

Format: Affinity-purified

Description: All names and symbols: MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Mdm2. Official Symbol: MDM2. RefSeq number(s): NP_002383.2

Specificity: This antibody is expected to recognize isoform MDM2 (NP_002383.2) only. Human, Dog, Cat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2). Recommended concentration: 0.3-1.0µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185508 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Bond, Hu, Bond et al.: "A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans." in: Cell, Vol. 119, Issue 5, pp. 591-602, 2004 (PubMed).