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Details for Product No. ABIN185543

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody

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Antigen
Synonyms
G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN185543
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037.2
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 36 kDa band observed in Human Liver, Testis and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1 kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Vugler, Carr, Lawrence et al.: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, 2008 (PubMed).

Carr, Vugler, Lawrence et al.: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, 2009 (PubMed).

Chakir, Daya, Aiba et al.: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, 2009 (PubMed).

Schillace, Miller, Pisenti et al.: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, 2009 (PubMed).

Carr, Vugler, Yu et al.: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, 2011 (PubMed).

Ismail, Park: "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase." in: Acta crystallographica. Section D, Biological crystallography, Vol. 61, Issue Pt 11, pp. 1508-13, 2005 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (250), (200), (36), (22), (20)
Reactivities (398), (245), (212), (108), (59), (39), (38), (36), (27), (26), (24), (20), (16), (14), (13), (11), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (237), (140), (81), (79), (60), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (24), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (82), (34), (10), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
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