Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody

Details for Product No. ABIN185543
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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Epitope
Internal Region
(85), (38), (14), (13), (10), (9), (8), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(403), (219), (196), (95), (39), (30), (28), (27), (17), (17), (16), (13), (12), (12), (9), (9), (8), (8), (7), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Goat
(276), (194), (33), (18), (17)
Clonality
Polyclonal
Conjugate
Un-conjugated
(31), (28), (12), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(451), (227), (128), (78), (76), (74), (49), (31), (17), (10), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 100 µg
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Catalog No. ABIN185543
374.27 $
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Immunogen Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 36 kDa band observed in Human Liver, Testis and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1 kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody ABIN185543 (0.1µg/ml) staining of Human Tonsil Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody (2) ABIN185543 (0.1 µg/mL) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody (3) ABIN185543 (0.5 µg/mL) staining of Rat Liver lysate (35 µg protein in RIPA buffer). Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody (4) ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I . Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Product cited in: Vugler, Carr, Lawrence et al.: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, 2008 (PubMed).

Carr, Vugler, Lawrence et al.: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, 2009 (PubMed).

Chakir, Daya, Aiba et al.: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, 2009 (PubMed).

Schillace, Miller, Pisenti et al.: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, 2009 (PubMed).

Carr, Vugler, Yu et al.: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, 2011 (PubMed).

General Ismail, Park: "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase." in: Acta crystallographica. Section D, Biological crystallography, Vol. 61, Issue Pt 11, pp. 1508-13, 2005 (PubMed).

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