anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Internal Region)

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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Epitope
Internal Region
(99), (51), (22), (16), (11), (10), (9), (9), (8), (7), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(574), (232), (202), (118), (61), (50), (46), (38), (28), (25), (21), (19), (18), (17), (12), (12), (10), (9), (9), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Goat
(346), (254), (61), (31), (21)
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
(43), (40), (18), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(624), (291), (165), (130), (93), (69), (47), (31), (28), (17), (10), (10), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Quantity 100 μg
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Immunogen Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.
Blocking Peptide Blocking peptide for this product available: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) peptide (ABIN369144)
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 36 kDa band observed in Human Liver, Testis and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1 kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Internal Region) (ABIN185543) ABIN185543 (0.1µg/ml) staining of Human Tonsil Lysate (35µg protein in RIPA buffer). ...
Image no. 2 for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Internal Region) (ABIN185543) ABIN185543 (0.1 µg/mL) staining of Human Liver lysate (35 µg protein in RIPA buffer)....
Image no. 3 for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Internal Region) (ABIN185543) ABIN185543 (0.5 µg/mL) staining of Rat Liver lysate (35 µg protein in RIPA buffer). P...
Image no. 4 for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Internal Region) (ABIN185543) ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver. Antigen retr...
Product cited in: Carr, Vugler, Yu et al.: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, 2011 (PubMed).

Schillace, Miller, Pisenti et al.: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, 2009 (PubMed).

Chakir, Daya, Aiba et al.: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, 2009 (PubMed).

Carr, Vugler, Lawrence et al.: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, 2009 (PubMed).

Vugler, Carr, Lawrence et al.: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, 2008 (PubMed).

Background publications Ismail, Park: "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase." in: Acta crystallographica. Section D, Biological crystallography, Vol. 61, Issue Pt 11, pp. 1508-13, 2005 (PubMed).

Catalog No. ABIN185543
$221.76
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