Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody

Details for Product No. ABIN185544, Supplier: Log in to see
Antigen
  • VPS25
  • CG14750
  • Dmel\\CG14750
  • ESCRT-I
  • ESCRT-II
  • dVps25
  • l(2)44Db
  • l(2)k08904
  • vps25
  • GB16268
  • derp9
  • eap20
  • fap20
  • DDBDRAFT_0189483
  • DDBDRAFT_0234026
  • DDB_0189483
  • DDB_0234026
  • 1110020N13Rik
  • AW107467
  • D11Wsu68e
  • im:6898134
  • zgc:162560
  • DERP9
  • EAP20
  • FAP20
  • vacuolar protein sorting 25 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 25
  • Vacuolar protein sorting 25
  • vacuolar protein sorting 25
  • vacuolar protein sorting 25 homolog
  • Vacuolar protein-sorting-associated protein 25
  • vacuolar protein sorting 25 (yeast)
  • VPS25
  • CpipJ_CPIJ010292
  • LOC100221661
  • Vps25
  • vps25
Epitope
Internal Region
18
13
11
5
3
3
1
1
Reactivity
Human
44
27
9
3
2
1
1
1
1
1
1
1
1
Host
Goat
36
15
4
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
49
37
23
8
3
1
1
Options
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Available images

Immunogen Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.
Blocking Peptide Blocking peptide for this product available: ABIN369679
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name VPS25 (VPS25 Antibody Abstract)
Background Synonyms: VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), FAP20
Gene ID 84313
NCBI Accession NP_115729
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration: 10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody (ABIN185544) ABIN185544 (0.5 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer)....
Immunohistochemistry (IHC) image for anti-Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody (ABIN185544) ABIN185544 (10 ug/ml) staining of paraffin embedded human kidney. Microwaved antigen ...
Background publications Vaccari, Bilder: "The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking." in: Developmental cell, Vol. 9, Issue 5, pp. 687-98, 2005 (PubMed).

Thompson, Mathieu, Sung, Loeser, Rorth, Cohen: "Tumor suppressor properties of the ESCRT-II complex component Vps25 in Drosophila." in: Developmental cell, Vol. 9, Issue 5, pp. 711-20, 2005 (PubMed).