UPF2 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF2) antibody

Antigen: UPF2 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF2)

Synonyms: HUPF2, RENT2, smg-3, KIAA1408, MGC138834, MGC138835, DKFZp434D222

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185547

Additional Information

Alternative name: RENT2 / UPF2

Gene ID: 26019

Immunogen: C-PRYQHPKGAPNAD

Description: All names and symbols: UPF2, UPF2 regulator of nonsense transcripts homolog (yeast), HGNC:17854, DKFZP434D222, HUPF2, KIAA1408, RENT2 , OTTHUMP00000045025, UPF2 regulator of nonsense transcripts homolog, regulator of nonsense transcripts 2, yeast Upf2p homolog. Official Symbol: UPF2. RefSeq number(s): NP_542166.1, NP_056357.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_542166.1, NP_056357.1). Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human HeLa lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Hosoda, Kim, Lejeune et al.: "CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells." in: Nature structural & molecular biology, Vol. 12, Issue 10, pp. 893-901, 2005 (PubMed).