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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Internal Region) antibody

Details for Product No. ABIN185582
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Antigen
Synonyms
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, IS ... show more
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, ISG12A, P27, 1500011J20Rik, 2610202L11Rik, Bridge-1 show less
Epitope
Internal Region
(69), (52), (35), (34), (28), (21), (12), (11), (10), (10), (9), (9), (9), (7), (7), (6), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(412), (182), (145), (81), (61), (54), (36), (13), (8), (3), (3), (1), (1), (1), (1)
Host
Goat
(384), (64), (13), (1), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(21), (19), (17), (11), (11), (11), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(300), (186), (91), (89), (60), (60), (51), (28), (13), (10), (6), (6), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185582
374.27 $
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Immunogen Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cat (Feline), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KIP1 / CDKN1B
Background Synonyms: CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1), CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B
Gene ID 1027
NCBI Accession NP_004055.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26 kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102(39):14028-33, PMID: 16169901). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Internal Region) antibody ABIN185582 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Baldassarre, Belletti, Nicoloso et al.: "p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion." in: Cancer cell, Vol. 7, Issue 1, pp. 51-63, 2005 (PubMed).

Validation Images
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