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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Internal Region) antibody

Antigen

Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyms KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B
Binding Site
Alternatives

Internal Region

Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN185582
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information

Alternative name KIP1 / CDKN1B
Gene ID 1027
Immunogen C-KRPATDDSSTQNK
Description All names and symbols: CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) , CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B. Official Symbol: CDKN1B. RefSeq number(s): NP_004055.1
Specificity Human, Dog, Cat 198 aa

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102(39):14028-33, PMID: 16169901). Recommended concentration: 0.1-0.3µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Baldassarre, Belletti, Nicoloso et al.: "p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion." in: Cancer cell, Vol. 7, Issue 1, pp. 51-63, 2005 (PubMed).

Alternatives

Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
Hosts Rabbit (108), Mouse (16), Goat (8)
Reactivities Human (113), Rat (Rattus) (60), Mouse (Murine) (58), Dog (Canine) (49), Pig (Porcine) (38), Cow (Bovine) (24), Chicken (20), Guinea Pig (15), Horse (Equine) (15), Sheep (Ovine) (10), Cat (Feline) (1), Hamster (1)
Applications Western Blotting (WB) (73), Immunofluorescence (IF) (44), ELISA (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunohistochemistry (IHC) (20), Immunoprecipitation (IP) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (4), FITC (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pThr187 (13), C-Term (11), pSer10 (10), pThr157 (7), pSer178 (6), pThr198 (6), Internal Region (1), pSer204 (1), pSer345 (1), pSer990 (1), pThr181 (1)