Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (internal) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185582

Additional Information

Alternative name: KIP1 / CDKN1B (internal)

Gene ID: 1027

Immunogen: C-KRPATDDSSTQNK

Format: Affinity-purified

Description: All names and symbols: CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) , CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B. Official Symbol: CDKN1B. RefSeq number(s): NP_004055.1

Specificity: Human, Dog, Cat 198 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102(39):14028-33, PMID: 16169901). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185582 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Baldassarre, Belletti, Nicoloso et al.: "p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion." in: Cancer cell, Vol. 7, Issue 1, pp. 51-63, 2005 (PubMed).