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LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (Internal Region)

Details for Product anti-LEF1 Antibody No. ABIN185607, Supplier: Log in to see
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Alternatives
anti-Human LEF1 antibody for Enzyme Immunoassay
Epitope
Internal Region
15
14
12
9
6
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
123
41
32
10
8
8
5
5
4
4
4
4
2
2
2
1
1
Host
Goat
55
48
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
112
64
21
13
7
5
3
2
2
2
1
1
1
1
Options
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Immunogen Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.
Blocking Peptide Blocking peptide for this product available: ABIN369644
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Synonyms: LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Gene ID 51176, 16842, 161452
NCBI Accession NP_057353
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (Internal Region) (ABIN185607) ABIN185607 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Prim...
Background publications Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).

Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).