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Cholinergic Receptor, Muscarinic 1 (CHRM1) (C-Term) antibody

Details for Product No. ABIN185701, Supplier: Login to see
Antigen
  • M1
  • CHRM1
  • HM1
  • M1R
  • mAChR
  • Chrm-1
Epitope
C-Term
19
13
5
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
60
55
35
2
1
Host
Goat
69
11
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
60
35
18
11
7
2
1
1
Supplier
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Immunogen Peptide with sequence C-DKRRWRKIPKRPGSVH, from the C Terminus of the protein sequence according to NP_000729.2.
Blocking Peptide Blocking peptide for this product available: ABIN368881
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name M1 mAChR / CHRM1 (CHRM1 Antibody Abstract)
Background Synonyms: CHRM1, M1 mAChR, cholinergic receptor, muscarinic 1, HM1, M1, MGC30125, muscarinic acetylcholine receptor M1
Gene ID 1128, 12669, 25229
NCBI Accession NP_000729
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50 kDa band observed in Mouse Heart and Rat Heart lysates (calculated MW of 51.4 kDa according to NP_000729.2). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 0.5mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Cholinergic Receptor, Muscarinic 1 (CHRM1) (C-Term) antibody (ABIN185701) ABIN185701 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Pri...
 image for anti-Cholinergic Receptor, Muscarinic 1 (CHRM1) (C-Term) antibody (ABIN185701) ABIN185701 (0.3 µg/mL) staining of Mouse Heart lysate (35 µg protein in RIPA buffer)....
Background publications Caccamo, Oddo, Billings et al.: "M1 receptors play a central role in modulating AD-like pathology in transgenic mice." in: Neuron, Vol. 49, Issue 5, pp. 671-82, 2006 (PubMed).