CHRM1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7043060

Product Details anti-CHRM1 Antibody

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Binding Specificity: 3rd Intracellular Loop, AA 227-353

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Anti-CHRM1 Antibody is directed against the 3rd intracellular loop of the human M1 muscarinic receptor. Anti-CHRM1 Antibody (ABIN7043060, ABIN7044580 and ABIN7044581)) can be used in western blot analysis, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize M1 from human, mouse and rat samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence GSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRK, corresponding to amino acid residues 227-353 of human M1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.95 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRM1

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Alternative Name: CHRM1 (CHRM1 Products)

Synonyms: M1 antibody, CHRM1 antibody, HM1 antibody, M1R antibody, mAChR antibody, Chrm-1 antibody, cholinergic receptor muscarinic 1 antibody, cholinergic receptor, muscarinic 1 antibody, cholinergic receptor, muscarinic 1, CNS antibody, CHRM1 antibody, Chrm1 antibody

Background: Alternative names: M1 Muscarinic Receptor, CHRM1, Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1

Gene ID: 1128

NCBI Accession: NM_000738

UniProt: P11229

Pathways: Synaptic Membrane