MUC16 antibody (AA 21622-21762) (Biotin)
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- Target See all MUC16 (CA125) Antibodies
- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
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Binding Specificity
- AA 21622-21762
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MUC16 antibody is conjugated to Biotin
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Sequence
- MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- RVIALRSVK NGAETRVDLL CTYLQPLSGP GLPIKQVFHE LSQQTHGITR LGPYSLDKDS LYLNGYNEPG LDEPPTTPKP ATTFLPPLSE ATTAMGYHLK TLTLNFTISN LQYSPDMGKG SATFNSTEGV LQHLLRPLFQ KS
- Specificity
- It has been selected for its ability to recognize CA125 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant CA125 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CA125 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product CA125 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant CA125 (Arg21622~Ser21762) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
- Alternative Name
- Carbohydrate Antigen 125 (CA125) (CA125 Products)
- Synonyms
- CA125 antibody, 1110008I14Rik antibody, Ca125 antibody, Gm1480 antibody, RGD1308422 antibody, MUC16 antibody, mucin 16, cell surface associated antibody, mucin 16 antibody, mucin-16 antibody, MUC16 antibody, Muc16 antibody, LOC476718 antibody, LOC712735 antibody
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