CD40 Ligand antibody (AA 105-260)
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- Target See all CD40 Ligand (CD40LG) Antibodies
- CD40 Ligand (CD40LG)
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Binding Specificity
- AA 105-260
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This CD40 Ligand antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)
- Sequence
- MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - KKEKSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVVLENGRQL TVKREGLYYV YTQVTFCSNR EPLSQRPFIV SLWLKPSSGS ERILLRAANT HSSSKLCEQQ SIHLGGVFEL QAGASVFVNV TEASQVIHGI GFSSIGLLKL
- Specificity
- The antibody is a mouse monoclonal antibody raised against CD40L. It has been selected for its ability to recognize CD40L in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Cluster Of Differentiation 40 Ligand (CD40L) corresdonding to Lys105~Leu260 (Accession # Q9Z2V2) with N-terminal His and GST Tag
- Clone
- C1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CD40LG Primary Antibody
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- Application Notes
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Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- CD40 Ligand (CD40LG Products)
- Synonyms
- CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody
- Background
- CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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