beta 2 Defensin antibody (AA 23-63)

Catalog No. ABIN1858624

Product Details anti-beta 2 Defensin Antibody

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Binding Specificity: AA 23-63

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta 2 Defensin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Defensin Beta 2 (DEFb2)

Specificity: The antibody is a rabbit polyclonal antibody raised against DEFb2. It has been selected for its ability to recognize DEFb2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Defensin Beta 2 (DEFb2) corresdonding to Gln23~Lys63 (Accession # O88514) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-beta 2 Defensin antibody (ABIN1858624)

Han, Kim, Ryu, Jang, Cha, Kim, Lee, Back, Kim, Na: "Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis." in: Journal of dermatological science, Vol. 90, Issue 3, pp. 276-283, (2018) (PubMed).

Target Details for beta 2 Defensin

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Alternative Name: Defensin Beta 2 (BD-2 Products)

Synonyms: BD-2 antibody, BD-4 antibody, DEFB-4 antibody, hBD-4 antibody, BD2 antibody, DEFB2 antibody, defensin beta 2 antibody, defensin beta 104B antibody, beta defensin 2 antibody, Defb2 antibody, DEFB104B antibody, SBD2 antibody

Background: B-DF2, DEFB2, BD2, DEF-B2, HBD2, DEFB102, HBD-2, SAP1, DEFB4A, DEFB4, Skin-Antimicrobial Peptide 1