FGF2 antibody (Fibroblast Growth Factor 2 (Basic)) (AA 26-153)

Details for Product anti-FGF2 Antibody No. ABIN1858868, Supplier: Log in to see
Antigen
  • Fgf-2
  • Fgfb
  • bFGF
  • FGF-2
  • BFGF
  • FGFB
  • HBGF-2
  • fibroblast growth factor 2
  • fibroblast growth factor 2 (basic)
  • Fgf2
  • fgf2
  • FGF2
Alternatives
anti-Cow (Bovine) FGF2 antibody for Immunohistochemistry
Epitope
AA 26-153
16
16
12
10
10
8
7
5
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rabbit
327
73
60
38
24
20
4
3
2
2
2
2
1
1
Host
Guinea Pig
196
154
8
5
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
33
19
13
9
6
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
263
217
101
63
32
27
26
19
15
13
13
13
11
7
7
4
4
2
2
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen FGF2 (AA 26-153)
Sequence MGHHHHHHSG SEF- FKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSA
Isotype IgG
Specificity It has been selected for its ability to recognize FGF2 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Alternative Name Fibroblast Growth Factor 2, Basic (FGF2) (FGF2 Antibody Abstract)
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant FGF2 (Phe26~Ala153) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 26-153) antibody (ABIN1858868) anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 26-153) antibody
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