TNC antibody (AA 49-181)

Catalog No. ABIN1860784

Product Details anti-TNC Antibody

Target: TNC (Tenascin C (TNC))

Binding Specificity: AA 49-181

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Tenascin C (TNC)

Sequence: MGHHHHHHSG SEF-VF NHVYNIKLPV GSQCSVDLES ASGEKDLAPP SEPSESFQEH TVDGENQIVF THRINIPRRA CGCAAAPDVK ELLSRLEELE NLVSSLREQC TAGAGCCLQP ATGRLDTRPF CSGRGNFSTE GCGCVCEPGW K

Specificity: The antibody is a mouse monoclonal antibody raised against TNC. It has been selected for its ability to recognize TNC in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tenascin C (TNC) corresdonding to Val49~Lys181 with N-terminal His Tag

Clone: C4

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-TNC antibody (ABIN1860784)

Gupta, Polisetty, Sharma, Ganesh, Gowda, Purohit, Ankathi, Prasad, Mariswamappa, Lakshmikantha, Uppin, Sundaram, Gautam, Sirdeshmukh: "Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion." in: Scientific reports, Vol. 9, Issue 1, pp. 10986, (2020) (PubMed).

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin C (TNC Products)

Synonyms: tn antibody, 150-225 antibody, GMEM antibody, GP antibody, HXB antibody, JI antibody, TN antibody, TN-C antibody, AI528729 antibody, C130033P17Rik antibody, Hxb antibody, Ten antibody, cytotactin antibody, tenascin-C antibody, P230 antibody, tenc antibody, wu:fk04d02 antibody, tenascin C L homeolog antibody, tenascin C antibody, tnc.L antibody, TNC antibody, MCYG_00667 antibody, tnc antibody, Tnc antibody

Background: TN-C, HXB, TN, GMEM, JI, Myotendinous antigen, Neuronectin, GP 150-225, Cytotactin, Hexabrachion, Glioma-associated-extracellular matrix antigen

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development