CYP27B1 antibody (AA 251-500)
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- Target See all CYP27B1 Antibodies
- CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
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Binding Specificity
- AA 251-500
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CYP27B1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against CYP27B1. It has been selected for its ability to recognize CYP27B1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Cytochrome P450 27B1 (CYP27B1) corresdonding to Asp251~Asp500 (Accession # O35132)
- Isotype
- IgG
- Top Product
- Discover our top product CYP27B1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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-
Lead Affects Vitamin D Metabolism in Rats." in: Nutrients, Vol. 10, Issue 3, (2018) (PubMed).
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Lead Affects Vitamin D Metabolism in Rats." in: Nutrients, Vol. 10, Issue 3, (2018) (PubMed).
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- Target
- CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
- Alternative Name
- Cytochrome P450 27B1 (CYP27B1 Products)
- Synonyms
- cp2b antibody, cyp1 antibody, cyp1alpha antibody, cyp27b antibody, p450c1 antibody, pddr antibody, vdd1 antibody, vddr antibody, vddri antibody, vdr antibody, CP2B antibody, CYP1 antibody, CYP1alpha antibody, CYP27B antibody, P450c1 antibody, PDDR antibody, VDD1 antibody, VDDR antibody, VDDRI antibody, VDR antibody, Cyp40 antibody, Cp2b antibody, Cyp1 antibody, Cyp27b antibody, Pddr antibody, Vdd1 antibody, Vddr antibody, VddrI antibody, Vdr antibody, cytochrome P450 family 27 subfamily B member 1 antibody, cytochrome P450, family 27, subfamily B, polypeptide 1 antibody, cytochrome P450, family 27, subfamily b, polypeptide 1 antibody, cyp27b1 antibody, CYP27B1 antibody, Cyp27b1 antibody
- Background
- VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase
- Pathways
- Metabolism of Steroid Hormones and Vitamin D
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