ITK antibody (AA 368-625)
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- Target See all ITK Antibodies
- ITK (IL2-Inducible T-Cell Kinase (ITK))
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Binding Specificity
- AA 368-625
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ITK antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to IL2 Inducible T-Cell Kinase (ITK)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against ITK. It has been selected for its ability to recognize ITK in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant IL2 Inducible T-Cell Kinase (ITK) corresdonding to Leu368~Leu625 with N-terminal His and GST Tag
- Isotype
- IgG
- Top Product
- Discover our top product ITK Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:250-1000 Immunohistochemistry: 5-20 μg/mL
1:25-100 Immunocytochemistry: 5-20 μg/mL
1:25-100 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- ITK (IL2-Inducible T-Cell Kinase (ITK))
- Alternative Name
- IL2 Inducible T-Cell Kinase (ITK Products)
- Synonyms
- Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody
- Background
- PSCTK2, EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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