ABCC5 antibody

Catalog No. ABIN187354

Product Details anti-ABCC5 Antibody

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ABCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: M5II-54 reacts with an internal epitope of human MRP5, a 190 - 200 kDa transmembrane protein that is closely related to the multi-drug resistance protein MRP. M5II-54 does not cross-react with the human MDR1, MRP1, MRP2, or MRP3 gene products. Species Reactivity: Human. Others not tested.

Sterility: 0.22 μm filtered

Immunogen: A bacterial fusion protein containing amino acids 722-910 of human MRP5.Lymph node cells from an immunized rat fused with SP2/O mouse myeloma cells.

Clone: M5II-54

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Optimal conditions still to be defined. Immunocytochemistry: Use a 1:20 to 1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-rat IgG and monoclonal rat APAAP complex. Alternatively, after incubation with primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use a 1:20 to 1:50 dilution (with anti-rat-HRP). The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL (> 200 tests) or 0.5 mL (> 100 tests) in serum-free culture supernatant at a concentration of 250 µg Ig/mL (concentration by ELISA), with 0.1% sodium azide and protein stabilizer. Sample has been filtered through a 0.22 µm filter. Culture Medium: I

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for ABCC5

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Alternative Name: ABCC5 / MRP5 (ABCC5 Products)

Synonyms: ABC33 antibody, EST277145 antibody, MOAT-C antibody, MOATC antibody, MRP5 antibody, SMRP antibody, pABC11 antibody, 2900011L11Rik antibody, AI132311 antibody, Abcc5a antibody, Abcc5b antibody, Mrp5 antibody, abc33 antibody, abcc5 antibody, moat-c antibody, moatc antibody, mrp5 antibody, pabc11 antibody, smrp antibody, ATP binding cassette subfamily C member 5 antibody, ATP-binding cassette, sub-family C (CFTR/MRP), member 5 antibody, ATP binding cassette subfamily C member 5 L homeolog antibody, ABCC5 antibody, Abcc5 antibody, abcc5.L antibody

Pathways: Glycosaminoglycan Metabolic Process