BMF antibody

Catalog No. ABIN187548

Product Details anti-BMF Antibody

Target: BMF (Bcl2 Modifying Factor (BMF))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This BMF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: Recognizes human and mouse Bmf. Species Reactivity: Human and mouse. Others not tested.

Immunogen: Recombinant mouse Bmf fused to glutathione-S-transferase (GST).

Clone: 12E10

Isotype: IgG1

Application Details

Application Notes: Western blot: Use at 0.5-1.0 µg/mL Flow cytometry: Use at 1-10 µg/mL (1:100- 1:1,000 dilution) Immunoprecipitation: Use at 2-5 µg/mL The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µg of protein G purified antibody in PBS containing 0.02% sodium azide. Concentration is 1 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for BMF

Target: BMF (Bcl2 Modifying Factor (BMF))

Alternative Name: BMF (BMF Products)

Synonyms: BMF antibody, AW260063 antibody, AW411872 antibody, Bcl2 modifying factor antibody, Bcl2 modifying factor L homeolog antibody, BCL2 modifying factor antibody, BMF antibody, bmf.L antibody, Bmf antibody

Background: Bmf is a novel BH3-only protein that was discovered in a yeast 2-hybrid library screen with Mcl-1 (a pro-survival member of the Bcl-2 family) as the target molecule. Biochemical experiments demonstrated that Bmf binds to Bcl-2 and all of its pro-survival homologs through its BH3 domain. Co-immunoprecipitation of Bmf and Bcl-2 can be detected in cells that express both proteins at physiological levels. Bmf promotes apoptosis by blocking the pro-survival activity of Bcl-2 and its homologs. In healthy cells, Bmf is sequestered to the actin cytoskeleton, via dynein light chain 2 (DLC2), allowing it to translocate and bind Bcl-2 and its homologs.