Aggrecan antibody
-
- Target See all Aggrecan (ACAN) Antibodies
- Aggrecan (ACAN)
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Aggrecan antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- Reacts with chondroitin sulfate proteoglycan from cartilage (aggrecan). Species Reactivity: Reacts with human. No crossreactivity with bovine nasal cartilage, rat chondrosarcoma, chick or shark cartilage aggrecan. Other species not tested. Controls: Purified antigen, cartilage extracts or mature cultured chondrocytes.
- Immunogen
- Human large molecular weight proteoglycan of articular cartilage (aggrecan).
- Clone
- 6F4
- Isotype
- IgG1
- Top Product
- Discover our top product ACAN Primary Antibody
-
-
- Application Notes
- ELISA: 1:500-1:1,000 (highest ELISA reactivity of our 3 anti-chondroitin/keratan sufate proteoglycans). In solid phase immunoassays, endo-beta-galactosidase digestion of aggrecan increases the immunoreactivity of the antibody. Also reacts with papain-digested aggrecan. Immunohistochemistry: (paraffin embedded): 1:50-1:3,000. Treat tissue sections with 2 mg/ml testicular hyaluronidase in 50 mM Na-acetate buffer, pH 5.5 for 1 hr at 37C, followed by treatment with 0.2 mg/ml Protease XXIV in TBS, pH 7.2, for the same time and temperature. Immunoprecipitation: 1:50-1:300 Western blotting: 1:100-1:300 Reacts with 3 trypsin-generated fragments of human cartilage aggrecan (prior digested with chondroitinase and endo-beta-galactosidase) which have apparent MW of 55, 65 and 95 kDa. The optimal dilution for a specific application should be determined by the researcher.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 µl ascites containing 0.01% sodium azide. Antibody concentration >1 mg/ml.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- Aggrecan (ACAN)
- Alternative Name
- Aggrecan (ACAN Products)
- Synonyms
- AGC1 antibody, AGCAN antibody, CSPG1 antibody, CSPGCP antibody, MSK16 antibody, SEDK antibody, Agc antibody, Agc1 antibody, Cspg1 antibody, b2b183Clo antibody, cmd antibody, aggrecan antibody, aggrecan core protein antibody, ACAN antibody, Acan antibody, LOC100380783 antibody, AGC antibody
- Pathways
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
-