ASAH1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid freezing and thawing repeatly.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term use.Store at -20 °C for long term preservation.
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids.These ceramidases are classified according to the pH range that supports their optimal activity.ASAH1 is an acid ceramidase and key regulator of ceramide metabolism.Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice.Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide.Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis.Multiple isoforms of this protein are known to exist. Synonyms: N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 1, acid ceramidase