PTRH2
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid freezing and thawing repeatly.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term use.Store at -20 °C for long term preservation.
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading.Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment.Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process.Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis.Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination.It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE.Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin. Synonyms: Bcl-2 inhibitor of transcription 1, Peptidyl-tRNA hydrolase 2