At least four isoforms of DPAGT1 are known to exist, this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
Purification
Affinity chromatography purified via peptide column
Immunogen
17 amino acid peptide near the amino terminus of human DPAGT1.
DPAGT1
Reactivity: Mouse, Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 555
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid freezing and thawing repeatly.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term use.Store at -20 °C for long term preservation.
The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis.Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia.Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression. Synonyms: DPAGT, DGPT, GPT, ALG7, G1PT, UAGT, UGAT, CDG1J, CDG-Ij, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase