PH Domain and Leucine Rich Repeat Protein Phosphatase Like (PHLPP2) antibody

Antigen: PH Domain and Leucine Rich Repeat Protein Phosphatase Like (PHLPP2)

Synonyms: PHLPPL, KIAA0931

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190753

Additional Information

Alternative name: PHLPP2

Gene ID: 23035

Immunogen: C-PHEEDRTEPPEEFD

Format: Affinity-purified

Description: All names and symbols: PHLPP2, PHLPPL, PH domain and leucine rich repeat protein phosphatase-like , KIAA0931. Official Symbol: PHLPPL. RefSeq number(s): NP_055835.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 140kDa band observed in Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysates (calculated MW of 134kDa according to NP_055835.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN190753 (0.1µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Gao, Furnari, Newton: "PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." in: Molecular cell, Vol. 18, Issue 1, pp. 13-24, 2005 (PubMed).