Neutrophil Cytosolic Factor 4 (NCF4) antibody

Antigen: Neutrophil Cytosolic Factor 4 (NCF4)

Synonyms: NCF, MGC3810, P40PHOX, SH3PXD4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190755

Additional Information

Alternative name: NCF4 / P40PHOX

Gene ID: 4689

Immunogen: C-KDFPEEDDPTN

Format: Affinity-purified

Description: All names and symbols: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa , CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD). Official Symbol: NCF4. RefSeq number(s): NP_000622.2, NP_038202.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Autophagy

Restrictions: For Research Use only

Images

ABIN190755 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing P40PHOX (RC201191) and probed with ABIN190755 (mock transfection in first lane), tested by Origene.

Publications

Publications: Rioux, Xavier, Taylor et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).