X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6)

Synonyms: ML8, KU70, TLAA, CTC75, CTCBF, G22P1, XRCC6, MGC82304, DKFZp459H0114

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190763

Additional Information

Alternative name: KU70 / XRCC6

Gene ID: 2547

Immunogen: YKTEGDEEAEEEQ-C

Format: Affinity-purified

Description: All names and symbols: XRCC6, KU70, X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa) , CTC75, CTCBF, G22P1, ML8, TLAA , ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, Ku autoantigen p70 subunit, OTTHUMP00000028581, thyroid autoantigen 70kD (Ku antigen), thyroid autoantigen 70kDa (Ku antigen), thyroid-lupus autoantigen p70. Official Symbol: XRCC6. RefSeq number(s): NP_001460.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 70kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approx. 70kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190763 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing KU70 (RC204048) and probed with ABIN190763 (mock transfection in first lane), tested by Origene.

Publications

Publications: Ribes-Zamora, Mihalek, Lichtarge et al.: "Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functions." in: Nature structural & molecular biology, Vol. 14, Issue 4, pp. 301-7, 2007 (PubMed).