XRCC6 antibody (C-Term)

Catalog No. ABIN2855186

There is 1 publication for this product available. The Rabbit Polyclonal anti-XRCC6 antibody is suitable to detect XRCC6 in samples from Human. It has been validated for WB, IF, IHC (p), IP, ICC and PLA.

Quick Overview for XRCC6 antibody (C-Term) (ABIN2855186)

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Product Details anti-XRCC6 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Ku70 (X-ray repair complementing defective repair in Chinese hamster cells 6)
Ku70 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-XRCC6 antibody (ABIN2855186)

Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: X-ray repair cross complementing 6

Background: The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Cellular Localization: Nucleus

Molecular Weight: 70 kDa

Gene ID: 2547

UniProt: P12956

Pathways: DNA Damage Repair