ROBO3 antibody (Internal Region)

Catalog No. ABIN190793

Product Details anti-ROBO3 Antibody

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: ROBO3 / RIG1 (internal)

Sequence: RRKQRKELSH YT

Predicted Reactivity: Human, Dog, Pig, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RRKQRKELSHYT, from the internal region of the protein sequence according to NP_071765.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 150 kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148 kDa according to NP_071765.2). Recommended concentration: 0.5-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ROBO3 antibody (ABIN190793)

Bauer, Dowejko, Bosserhoff, Reichert, Bauer: "Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line." in: Carcinogenesis, Vol. 32, Issue 6, pp. 935-43, (2011) (PubMed).

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3 (ROBO3 Products)

Synonyms: HGPPS antibody, HGPS antibody, RBIG1 antibody, RIG1 antibody, CG14345 antibody, CT17158 antibody, Robo-3 antibody, Robo3 antibody, dRobo-3 antibody, DmelCG5423 antibody, CG5423 antibody, ROBO3 antibody, robo3 antibody, DKFZp468G0223 antibody, Rbig1 antibody, Rig-1 antibody, Rig1 antibody, Robo3a antibody, Robo3b antibody, cb503 antibody, roundabout guidance receptor 3 antibody, roundabout 3 antibody, robo3 antibody, roundabout guidance receptor 3 L homeolog antibody, roundabout, axon guidance receptor, homolog 3 (Drosophila) antibody, ROBO3 antibody, robo3 antibody, Robo3 antibody, LOC658818 antibody, robo3.L antibody

Background: ROBO3, RIG1, roundabout, axon guidance receptor, homolog 3 (Drosophila) , FLJ21044, HGPPS, RBIG1, retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3

Gene ID: 64221

NCBI Accession: NP_071765