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Antibodies
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anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (internal) antibody
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (internal) antibody
| Antigen |
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| Synonyms |
PEPCK1, PEPCKC, PEPCK-C, MGC22652, cb856, cb924, fj93h11, zgc:63869, wu:fc51c05, wu:fj93h11, MGC68864, PCK1, PPCK1, DKFZp469B0620 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (24), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Enzyme Immunoassay (EIA) (10), ELISA (8), Immunohistochemistry (IHC) (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN190801 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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PCK1 / PEPCKC (internal region)
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Gene ID
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5105
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Immunogen
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C-EKEVEDIEKYLEDQ
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Format
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Affinity-purified
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Description
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All names and symbols: PCK1, PEPCKC , phosphoenolpyruvate carboxykinase 1 (soluble) , MGC22652, PEPCK1, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase. Official Symbol: PCK1. RefSeq number(s): NP_002582.2
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Specificity
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Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 69.2kDa according to NP_002582.2). Recommended concentration: 0.5-1.5µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Willer, Bonnycastle, Conneely et al.: "Screening of 134 single nucleotide polymorphisms (SNPs) previously associated with type 2 diabetes replicates association with 12 SNPs in nine genes." in: Diabetes, Vol. 56, Issue 1, pp. 256-64, 2006 (PubMed).
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Alternatives for antigen "phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1)", type "Antibodies"
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Hosts
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Rabbit (36), Goat (8), Mouse (4)
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Reactivities
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Human (41), Mouse (Murine) (23), Rat (Rattus) (22), Dog (Canine) (4), Guinea Pig (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1)
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Applications
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Western Blotting (WB) (24), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Enzyme Immunoassay (EIA) (10), ELISA (8), Immunohistochemistry (IHC) (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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internal (3), C-Term (2), N-Term (2)
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