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CLIC4 antibody (Chloride Intracellular Channel 4) (N-Term)

Details for Product anti-CLIC4 Antibody No. ABIN190810, Supplier: Log in to see
Antigen
  • cb142
  • CLIC4
  • clic4
  • CLIC4L
  • D0Jmb3
  • DKFZp459A0325
  • H1
  • huH1
  • mc3s5
  • MGC79614
  • MGC89006
  • mtCLIC
  • MTCLIC
  • p64H1
  • TU-74
  • wu:fb33c05
  • wu:fc30h10
  • xclic4
Epitope
N-Term
25
15
7
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
86
50
47
6
2
1
1
1
1
1
1
Host
Goat
75
18
5
Clonality
Polyclonal
Conjugate
This CLIC4 antibody is un-conjugated
8
5
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
72
50
32
22
18
11
5
3
3
1
Supplier
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Immunogen Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.
Blocking Peptide Blocking peptide for this product available: ABIN370258
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CLIC4 (CLIC4 Antibody Abstract)
Background Synonyms: CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1, chloride intracellular channel 4 like
Gene ID 25932, 29876, 83718
NCBI Accession NP_039234
Research Area Signaling, Metabolism, Cell Cycle, Apoptosis/Necrosis
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 30 kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8 kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar,276(3 Pt 2):F398-408., PMID: 10070163). Recommended concentration: 0.1-0.3 µg/mL. Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration: 5 µg/mL with overnight incubation.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-CLIC4 antibody (Chloride Intracellular Channel 4) (N-Term) (ABIN190810) ABIN190810 (0.1µg/ml) staining of human kidney lysate (35µg protein in RIPA buffer). ...
 image for anti-CLIC4 antibody (Chloride Intracellular Channel 4) (N-Term) (ABIN190810) ABIN190810 (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclea...
Background publications Suh, Crutchley, Koochek, Ryscavage, Bhat, Tanaka, Oshima, Fitzgerald, Yuspa: "Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 1, pp. 121-31, 2007