Dyskeratosis Congenita 1, Dyskerin (DKC1) (Internal Region) antibody

Details for Product No. ABIN190831, Supplier: Log in to see
Antigen
  • fv62a07
  • wu:fa28f10
  • wu:fc87a02
  • wu:fi24a05
  • wu:fv62a07
  • zgc:110395
  • DKC1
  • cbf5
  • dkc
  • nap57
  • nola4
  • xap101
  • CBF5
  • DKC
  • DKCX
  • NAP57
  • NOLA4
  • XAP101
  • BC068171
  • Nap57
  • dyskerin
  • AtCBF5
  • AtNAP57
  • homologue of NAP57
  • dyskeratosis congenita 1, dyskerin
  • dyskerin
  • putative pseudouridine synthase NAP57
  • DKC1
  • dkc1
  • LOC100340288
  • Dkc1
  • NAP57
Alternatives
anti-Human Dyskeratosis Congenita 1, Dyskerin antibody for ELISA
Epitope
Internal Region
24
10
10
8
4
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
89
53
36
4
4
3
3
3
3
2
2
1
1
1
1
1
Host
Goat
68
19
4
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
58
46
36
35
17
14
13
6
5
1
1
Options
Supplier
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Immunogen Peptide with sequence C-KRKRESESESDETPP, from the internal region of the protein sequence according to NP_001354.1.
Blocking Peptide Blocking peptide for this product available: ABIN369357
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DKC1 (DKC1 Antibody Abstract)
Background Synonyms: DKC1, dyskeratosis congenita 1, dyskerin, DKC, NAP57, NOLA4, XAP101
Gene ID 1736, 245474
NCBI Accession NP_001354
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA
Pathways Telomere Maintenance
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 70-75 kDa band in Human Bone Marrow, Duodenum and Skin lysates after 0.01 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7 kDa according to NP_001354.1. The 70-75 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Spleen shows strong nuclear staining of select splenocytes. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (Internal Region) antibody (ABIN190831) ABIN190831 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retri...
Background publications Yoon, Peng, Brandenburger, Brandenburg, Zollo, Xu, Rego, Ruggero: "Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita." in: Science (New York, N.Y.), Vol. 312, Issue 5775, pp. 902-6, 2006 (PubMed).