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CD6 antibody (CD6) (PE)

Details for Product anti-CD6 Antibody No. ABIN192058, Supplier: Log in to see
Antigen
  • OX52
  • TP120
Reactivity
Human
263
55
50
8
5
Host
Mouse
280
47
21
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD6 antibody is conjugated to PE
32
25
20
8
7
7
7
6
6
6
5
5
5
5
5
4
4
4
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
251
182
144
110
100
98
72
67
45
11
6
5
Supplier
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Immunogen Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).
Clone MEM-98
Isotype IgG1
Specificity The MEM-98 antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD6 (CD6 Antibody Abstract)
Background CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD6 antibody (CD6)  (PE) (ABIN192058) Surface staining of human peripheral blood cells with anti-human CD6 (MEM-98) FITC.
Product cited in: Castro, Oliveira, Nunes, Fabre, Barbosa, Peixoto, Brown, Parnes, Bismuth, Moreira, Rocha, Carmo: "Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4351-61, 2007 (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Background publications Hassan, Simmonds, Clarkson, Hanrahan, Puklavec, Bomb, Barclay, Brown: "CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6727-38, 2006 (PubMed).

Zimmerman, Joosten, Torensma, Parnes, van Leeuwen, Figdor: "Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells." in: Blood, Vol. 107, Issue 8, pp. 3212-20, 2006 (PubMed).

Osorio, De Santiago, Aguilar-Santelises, Mellstedt, Jondal: "CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM." in: Blood, Vol. 89, Issue 8, pp. 2833-41, 1997 (PubMed).