EpCAM antibody
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- Target See all EpCAM (EPCAM) Antibodies
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EpCAM antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- IHC-plus™
- Specificity
- Recognizes the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.
- Purification
- Purified IgG
- Immunogen
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HT29 carcinoma cell line.
Type of Immunogen: Cells - Clone
- HEA125
- Isotype
- IgG1
- Top Product
- Discover our top product EPCAM Primary Antibody
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- Application Notes
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Approved: Flo (1:1), IHC, IHC-Fr (1:20), IHC-P (1:50)
Usage: Flow Cytometry: Use 5 μL of the suggested working dilution to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. 0.1 % pronase, 10 minutes. Histology Positive Control Tissue: Human colon & appendix. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.3, 0.09 % sodium azide, 0.5 % BSA.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Alternative Name
- ESA / EPCAM (EPCAM Products)
- Synonyms
- DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
- Background
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Name/Gene ID: EPCAM
Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1 - Gene ID
- 4072
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