STAT5A antibody (pSer780)

Catalog No. ABIN196698

The Rabbit Polyclonal anti-STAT5A antibody (ABIN196698) specifically detects STAT5A in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for STAT5A antibody (pSer780) (ABIN196698)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAT5A Antibody

Binding Specificity: pSer780

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. STAT5A (phospho-Ser780) antibody detects endogenous levels of STAT5A only when phosphorylated at serine 780.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of serine 780 (R-L-SP-P-P).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5 / STAT5A

Background: Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A

Gene ID: 6776

NCBI Accession: NP_003143

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation