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STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pSer780)

Details for Product anti-STAT5A Antibody No. ABIN196698, Supplier: Login to see
Antigen
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
Alternatives
anti-Human STAT5A antibody for Immunohistochemistry (Frozen Sections)
Epitope
pSer780
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This STAT5A antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of serine 780 (R-L-SP-P-P).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. STAT5A (phospho-Ser780) antibody detects endogenous levels of STAT5A only when phosphorylated at serine 780.
Purification Immunoaffinity chromatography.
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Gene ID 6776
NCBI Accession NP_003143
UniProt P42229
Pathways JAK-STAT Signaling, RTK Signaling
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pSer780) (ABIN196698) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer780) antibody
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pSer780) (ABIN196698) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer780) antibody (Image 2)
Background publications Dentelli, Del Sorbo, Rosso et al.: "Human IL-3 stimulates endothelial cell motility and promotes in vivo new vessel formation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 2151-9, 1999 (PubMed).

Meinke, Barahmand-Pour, Wöhrl et al.: "Activation of different Stat5 isoforms contributes to cell-type-restricted signaling in response to interferons." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6937-44, 1997 (PubMed).

Gouilleux, Wakao, Mundt et al.: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994 (PubMed).