Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Integrin beta3 around the phosphorylation site of tyrosine 773 (P-L-YP-K-E).
Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Human Integrin beta 3 (CD61) antigen is a 90-110 kDa glycoprotein polypeptide which is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. The platelet glycoprotein is the integrin beta 3 chain of the vitronectin receptor and GPIIb/IIIa complex.Synonyms: GP3A, GPIIIa, Integrin beta-3, Platelet membrane glycoprotein IIIa