Progesterone Receptor antibody (pSer190)

Catalog No. ABIN196746

Product Details anti-Progesterone Receptor Antibody

Target: Progesterone Receptor (PGR)

Binding Specificity: pSer190

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Serine 190.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide

Immunogen: Synthetic phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of Serine 190 (G-L-Sp-P-A).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor (PGR Products)

Synonyms: NR3C3 antibody, PR antibody, 9930019P03Rik antibody, BB114106 antibody, ENSMUSG00000074510 antibody, PR-A antibody, PR-B antibody, XPR-1 antibody, pgr antibody, xpr-2 antibody, LOC443444 antibody, PGR antibody, pg antibody, pr antibody, gb:dq017620 antibody, progesterone receptor antibody, progesterone receptor L homeolog antibody, PGR antibody, Pgr antibody, pgr.L antibody, pgr antibody

Background: Progesteron receptors (PRs) are nuclear hormone receptors of the NR3C class, which also inculdes mineralocorticoid, glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-kappaB, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary fland and receptor expression is induced by estrogen. Well established functions of progesterone receptores include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition, progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory center to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and druing pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.Synonyms: NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR

Molecular Weight: 99 kDa

Gene ID: 5241

NCBI Accession: NP_000917

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration