Goat anti-Mouse IgG3 (Heavy Chain) Antibody (FITC)
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- Target See all IgG3 products
- IgG3
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), FLISA
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 0.5 % of target signal.
- Purification
- Immunoaffinity purified
- Immunogen
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Mouse IgG3 heavy chain.
Type of Immunogen: Purified protein
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- Application Notes
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Approved: ELISA, FLISA (1:10000 - 1:50000), Flo, IF (1:1000 - 1:5000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
Usage: Anti-Mouse IgG3 secondary antibody is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 BSA 10 mg/mL, 0.01 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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- Target
- IgG3
- Abstract
- IgG3 Products
- Target Type
- Antibody
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