LCK antibody (pTyr394)

Catalog No. ABIN196861

The Rabbit Polyclonal anti-LCK antibody (ABIN196861) specifically detects LCK in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for LCK antibody (pTyr394) (ABIN196861)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LCK Antibody

Binding Specificity: pTyr394

Specificity: This antibody detects endogenous levels of Lck only when phosphorylated at Tyrosine 394.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around the phosphorylation site of Tyrosine 394 (N-E-pY-T-A) derived from Human Lck.

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: LCK

Background: Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.Synonyms: LSK, Lymphocyte cell-specific protein-tyrosine kinase, Proto-oncogene tyrosine-protein kinase LCK, T cell-specific protein-tyrosine kinase, p56-LCK

Molecular Weight: 56 kDa

Gene ID: 3932

NCBI Accession: NP_001036236

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling