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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody

Details for Product No. ABIN196885
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Antigen
Synonyms zap70, MGC80670, MGC88962, ZAP70, 70kDa, AI327364, Srk, TZK, ZAP-70, mrtle, mur, SRK, STCD, STD
Epitope
pTyr319
(40), (38), (26), (19), (19), (14), (11), (10), (7), (7), (7), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(335), (159), (111), (49), (48), (37), (24), (24), (1)
Host
Rabbit
(239), (85), (8), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(17), (14), (11), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(209), (108), (96), (59), (50), (40), (27), (21), (16), (3), (2), (1)
Pubmed 5 references available
Quantity 50 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN196885
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Immunogen Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
Specificity This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 319.
Purification Immunoaffinity Chromatography
Alternative Name ZAP70
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Alternate names: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070.2
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody (2) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody (Image 2)
Background publications Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997 (PubMed).

Williams, Irvin, Sutor et al.: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999 (PubMed).

Tang, Sawasdikosol, Chang et al.: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Validation Images
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