Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN196885

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms SRK, STCD, STD, TZK, ZAP-70, Srk, zap70, MGC80670, MGC88962, ZAP70
Epitope
»Alternatives pTyr319
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN196885
Quantity 50 µg
Price
275.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
Specificity This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 319.
Purification Immunoaffinity Chromatography
Alternative Name ZAP70
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Alternate names: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070.2
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997 (PubMed).

Williams, Irvin, Sutor et al.: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999 (PubMed).

Tang, Sawasdikosol, Chang et al.: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts (212), (101), (8), (4), (1)
Reactivities (322), (161), (107), (49), (48), (37), (24), (24), (1), (1), (1), (1), (1)
Applications (191), (104), (66), (64), (61), (40), (34), (22), (18), (6), (2), (2), (2), (1)
Conjugates (16), (12), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Epitopes (34), (33), (20), (19), (18), (14), (8), (8), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon