Phosphotyrosine (phosphorylated) antibody (HRP)

Details for Product No. ABIN197634, Supplier: Log in to see
Antigen
Epitope
phosphorylated
25
19
1
1
1
1
1
1
Reactivity
Amino Acid
142
107
72
29
28
25
11
10
9
3
1
Host
Mouse
244
70
2
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
29
26
16
15
6
6
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
248
147
142
120
119
98
71
64
45
27
13
4
2
2
1
1
1
1
1
Options
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Immunogen U266 cell line.
Clone PY20
Isotype IgG2b
Purification Affinity chromatography
Target Type Amino Acid
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes ELISA: 1/1000 - 1/4000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 50 % PBS, pH 7.4 and 50 % Glycerol
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Ruff-Jamison, Campos-González, Glenney: "Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features." in: The Journal of biological chemistry, Vol. 266, Issue 10, pp. 6607-13, 1991 (PubMed).

Takagi, Daibata, Last, Humphreys, Parker, Sairenji: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).