Phosphotyrosine antibody

Catalog No. ABIN5775098

Product Details anti-Phosphotyrosine Antibody

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against Phosphotyrosine.

Specificity: The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.

Cross-Reactivity (Details): The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.

Immunogen: Phosphotyrosine containing carrier protein.

Clone: E10

Isotype: IgG1, IgG2a

Application Details

Application Notes: ELISA (0.1 μg/mL)
Flow Cytometry (4 μg/mL)
Immunocytochemistry (4 μg/mL)
Immunofluorescence (4 μg/mL)
Immunoprecipitation (1-4 μg/mL)
Western Blot (1-2 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C or lower.
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid