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Phosphotyrosine antibody

Name: Phosphotyrosine antibody
SKU: ABIN5774320
Price: 749.33 USD
Availability: InStock

WB, IP, IHC, FACS, ELISA, ICC Host: Rabbit Monoclonal RM111 unconjugated

Western blot analysis of serum-starved A431 cells nontreated or treated with 100 ng/mL EGF using Phosphotyrosine monoclonal antibody, clone RM111 at 1:5000 dilution. (Phosphotyrosine antibody)

Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with EGF (B) using Phosphotyrosine monoclonal antibody, clone RM111 (Red) at 1:500 dilution. (Phosphotyrosine antibody)

ICC

Immunoprecipitation analysis of Lane 1: EGE-treated A431 cell whole lysates, Lane 2: EGE-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: EGE-treated A431 cell whole lysates using Phosphotyrosine monoclonal antibody, clone RM111 at 1:1000 dilution. (Phosphotyrosine antibody)

3 images

Catalog No. ABIN5774320

Datasheet as PDF

Target See all Phosphotyrosine products

Phosphotyrosine

Reactivity

Please inquire
Host
89

23

1
Rabbit
Compatible Secondaries
Clonality
100

13
Monoclonal
Conjugate
41

16

10

7

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
Application
79

55

35

32

32

22

22

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Purpose

Recombinant rabbit monoclonal antibody raised against phosphotyrosine.

Specificity

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine

Cross-Reactivity (Details)

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine

Immunogen

Original antibody is raised against phosphotyrosine conjugated with BSA.

Clone

RM111

Isotype

IgG
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, FACS, ELISA, ICC Host: Mouse Monoclonal E10 unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 HRP

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM Host: Mouse Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 FITC

Application Notes

ELISA
Flow Cytometry
Immunocytochemistry (1:200-1:500)
Immunohistochemistry (1:200-1:500)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Target

Phosphotyrosine

Abstract

Phosphotyrosine Products

Target Type

Amino Acid