ITGA3 antibody

Catalog No. ABIN199324

Product Details anti-ITGA3 Antibody

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA3 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Specificity: Recognizes an epitope related to the VLA-3 complex (a3/b1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of 35S-methionine labeled KJ29 cells. Insolubilized antibody is capable of retaining both chains in a wide range of  pH values (2 to 10.5) with no preferential loss of the a3/b1 Molecule. Recognizes two chains with apparent MW of 110 and 150kD. In the presence of a reducing agent (2 % B-ME), a single MW of 130kD is detected.

Purification: Purified

Immunogen: Human renal carcinoma cell line (KJ29).

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, ICC, IP, RIA

Usage: Suitable for use in Immunohistochemistry, ELISA, RIA, Immunoprecipitation, Immunocytochemistry and FACS. Immunohistochemistry: Acetone fixed, paraffin embedded sections. Immunoprecipitation: Under non-reducing conditions, two chains with apparent molecular weight of 110 and 150kD. In the presence of a reducing agent (2 % B-ME), a single molecular species of 130kD is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight. Positive control: Cell Line: KJ29 renal carcinoma adherent cells.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: ITGA3 / CD49c (ITGA3 Products)

Synonyms: CD49C antibody, GAP-B3 antibody, GAPB3 antibody, ILNEB antibody, MSK18 antibody, VCA-2 antibody, VL3A antibody, VLA3a antibody, ITGA3 antibody, AA407068 antibody, integrin subunit alpha 3 antibody, integrin subunit alpha 3 S homeolog antibody, integrin alpha 3 antibody, ITGA3 antibody, itga3.S antibody, Itga3 antibody

Background: Name/Gene ID: ITGA3
Family: Integrin

Synonyms: ITGA3, Alpha3 integrin, CD49c antigen, CD49C, GAPB3, Integrin alpha-3, FRP-2, VCA-2, VLA-3 subunit alpha, VL3A, VLA3a, Galactoprotein B3, GAP-B3, ILNEB, Integrin alpha3, MSK18

Gene ID: 3675

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex