MDM2 antibody (pSer185)

Catalog No. ABIN201305

The Rabbit Polyclonal anti-MDM2 antibody is suitable to detect MDM2 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for MDM2 antibody (pSer185) (ABIN201305)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MDM2 Antibody

Binding Specificity: AA 177-198, pSer185

Specificity: Recognizes mouse MDM 2 showing a band at ~102kD corresponding to phosphorylated MDM2. Cellular Localization: Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleus. The nucleolar localization signals in both ARF and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to aa177-198, hrkrrrslsfdpslglcel of mouse MDM2.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:5000), WB (1:1000)

Usage: Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:5000. Western Blot: 1:1000.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background: Name/Gene ID: MDM2

Synonyms: MDM2, ACTFS, Double minute 2 protein, Hdm2, MDM2 variant FB30, HDMX, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2 variant FB28

Gene ID: 4193

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway