p21 antibody

Catalog No. ABIN206537

The Mouse Monoclonal anti-p21 antibody is suitable to detect p21 in samples from Human. It has been validated for WB and IP.

Quick Overview for p21 antibody (ABIN206537)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-p21 Antibody

Specificity: Recognizes the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1. The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli. Detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation.

Purification: Ammonium sulfate precipitation

Immunogen: Human p21 protein.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IP, WB (1:100 - 1:1000)

Usage: Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A / WAF1 / p21

Background: Name/Gene ID: CDKN1A

Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy