MGMT antibody

Catalog No. ABIN209041

This Mouse Monoclonal antibody specifically detects MGMT in WB, IHC, IF, FACS, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for MGMT antibody (ABIN209041)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-MGMT Antibody

Specificity: Recognizes MGMT in human tissues, tumors, cells or xenograft lines. Weak cross-reactivity with MGMT from mouse, hamster and rat cells.

Purification: Protein A purified

Immunogen: Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-P, IP, WB (1:500)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500 detects MGMT on 10 μg of Jurkat lysates. Immunohistochemistry: paraffin sections. Immunofluorescence (IC): HeLa cells. Positive control: Tonsil tissue, HeLa cells, CEM-CCRF cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Name/Gene ID: MGMT

Synonyms: MGMT

Gene ID: 4255

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus